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Faculty: Emery H. Bresnick, PhD

Director of UW-Madison Blood Research Program

Dept: Professor, Cell & Regenerative Biology
Director, UW Madison Blood Research Program
Contact: 4009 WIMR, L4
1111 Highland Ave
Training Areas:
  • Molecular & Env Toxicology
  • Biostatistics (Affiliate Trainer)
  • Molecular and Cellular Pharmacology
  • Cell & Molecular Biology
  • Biomolecular Chemistry
  • Cancer Biology
  • Biotechnology
  • Pathology & Laboratory Medicine
  • Hematology
  • MD/PhD
  • Translational Cardiovascular Science

Research Interests

Image related to research.

Figure 1

We use multidisciplinary approaches to understand stem/progenitor cell function, normal and malignant blood cell development, and vascular biology. Such approaches include genomics, proteomics, chemical genetics, embryonic stem cell differentiation, computational analysis, and traditional methodologies. In addition to elucidating biological principles, we aim to develop innovative therapeutic strategies based on targeting novel mechanisms.

Mechanisms of hematopoiesis:

We are analyzing the function/regulation of GATA factors that control hematopoietic stem cells (HSCs) and hematopoiesis. Transcriptional profiling and ChIP-seq analysis have identified a cohort of novel GATA factor target genes, including genes encoding proteins that bear no obvious similarity to known proteins. Loss-of-function and gain-of-function studies are being conducted in mice, zebrafish, human adult stem/progenitors, and embryonic stem cell systems to discover new modes of controlling stem/progenitor cell function and to devise novel applications towards translational medicine. Defining novel hematopoietic mechanisms has enormous importance, as deviations from physiological programs yield leukemias, lymphomas, myelodysplasia, and additional blood disorders.

Vascular mechanisms and engineering:

We have developed a novel murine model characterized by severe hematopoietic stem/progenitor cell depletion and a severe vascular integrity defect. We are dissecting mechanisms underlying the establishment and maintenance of vascular integrity in normal and disease states. Understanding such mechanisms is essential for developing efficient approaches to promote the vascularization of engineered tissues, a limiting factor in regenerative medicine.

Go to the Additional Bresnick Website

Honors & Awards

Recent Invited Lectures

Recent Study Section Service

Other Links

Selected Publications (Find recent publications on PubMed)

  • Gao X, Johnson KD, Chang Y-I, Boyer ME, Dewey C, Zhang J, and Bresnick EH (2013). Gata2 cis-element requirement for hematopoietic stem cell generation in the mammalian embryo. J. Exp. Med. In press
  • Lee HY, Johnson KD, Fujiwara T, Boyer ME, Kim SI, Bresnick EH. Controlling hematopoiesis through sumoylation-dependent regulation of a GATA factor. Mol Cell. 2009 Dec 25;36(6):984-95. doi: 10.1016/j.molcel.2009.11.005.
  • FujiwaraT ,O'Geen H, Keles S, Blahnik K, Linnemann AK, Kang YA, Choi K, Farnham PJ, Bresnick EH. Discovering hematopoietic mechanisms through genome-wide analysis of GATA factor chromatin occupancy. Mol Cell. 2009 Nov 25;36(4):667-81. doi: 10.1016/j.molcel.2009.11.001.
  • Zeng X, Sanalkumar R,Bresnick EH, Li H, Chang Q, Keleş S. jMOSAiCS: joint analysis of multiple ChIP-seq datasets. Genome Biol. 2013 Apr 29;14:R38. [Epub ahead of print] PMID: 23844871 [PubMed - as supplied by publisher]
  • Katsumura KR, Devilbiss AW, Pope NJ, Johnson KD,Bresnick EH.Transcriptional Mechanisms Underlying Hemoglobin Synthesis. Cold Spring Harb Perspect Med. 2013 Jul 9. doi:pii: cshperspect.a015412v1. 10.1101/cshperspect.a015412. [Epub ahead of print]PMID: 23838521 [PubMed - as supplied by publisher]
  • Fujita R, Takayama-Tsujimoto M, Satoh H, Gutiérrez L, Aburatani H, Fujii S, Sarai A, Bresnick EH, Yamamoto M, Motohashi H. NF-E2 p45 Is Important for Establishing Normal Function of Platelets. Mol Cell Biol. 2013 Jul;33(14):2659-70. doi: 10.1128/MCB.01274-12. Epub 2013 May 6. PMID: 23648484 [PubMed - in process]
  • Hsu AP, Johnson KD, Falcone EL, Sanalkumar R, Sanchez L, Hickstein DD, Cuellar-Rodriguez J, Lemieux JE, Zerbe CS, Bresnick EH, Holland SM. GATA2 haploinsufficiency caused by mutations in a conserved intronic element leads to MonoMAC syndrome.Blood. 2013 May 9;121(19):3830-7, S1-7. doi: 10.1182/blood-2012-08-452763. Epub 2013 Mar 15. PMID: 23502222 [PubMed - indexed for MEDLINE]
  • Pope NJ, Bresnick EH. Establishment of a Cell Type-Specific Genetic Network by the Mediator Complex Component Med1. Mol Cell Biol. 2013 Mar 4 [Epub ahead of print] PMID: 23459945
  • Guiu J, Shimizu R, D'Altri T, Fraser ST, Hatakeyama J, Bresnick EH, Kageyama R, Dzierzak E, Yamamoto M, Espinosa L, Bigas A. Hes repressors are essential regulators of hematopoietic stem cell development downstream of Notch signaling. J Exp Med. 2013 Jan 14;210(1):71-84. doi: 10.1084/jem.20120993. Epub 2012 Dec 24. PMID 23267012
  • Johnson KD, Hsu AP, Ryu M-J, Wang J, Gao X, Boyer ME, Keles S, Lee ., Calvo KR, Zhang J, Holland SM, and Bresnick EH. (2012) Cis-element mutated in GATA2-dependent immunodeficiency governs hematopoiesis and vascular integrity. J. Clin. Invest. 122:3692-3704. PMCID: PMC3461907
  • Kang YA, Sanalkumar R, O'Geen H, Linnemann AK, Chang CJ, Bouhassira EE, Farnham PJ, Keles S, Bresnick EH (2012). Autophagy driven by a master regulator of hematopoiesis. Mol Cell Biol. 32:226-239. PMID: 22025678
  • Linnemann AK, O'Geen H, Keles S, Farnham PJ, Bresnick EH (2011). Genetic framework for GATA factor function in vascular biology. Proc Natl Acad Sci U S A. 108:13641-13646. PMID: 21808000
  • Chung D, Kuan PF, Li B, Sanalkumar R, Liang K, Bresnick EH, Dewey C, Kele_ S (2011). Discovering transcription factor binding sites in highly repetitive regions of genomes with multi-read analysis of ChIP-Seq data. PLoS Comput Biol. 7(7):e1002111. PMID: 21779159
  • Lee HY, Johnson KD, Boyer ME, Bresnick EH (2011). Relocalizing genetic loci into specific subnuclear neighborhoods. J Biol Chem. 286:18834-18844. PMID: 21398517
  • Fujiwara T, Lee HY, Sanalkumar R, Bresnick EH (2010). Building multifunctionality into a complex containing master regulators of hematopoiesis. Proc Natl Acad Sci U S A.107:20429-20434. PMID: 21059912
  • Bresnick EH, Lee HY, Fujiwara T, Johnson KD, and Keles S (2010). GATA switches as developmental drivers. J Biol Chem. 285:31087-93. PMID: 20670937
  • Fujiwara T, Lee HJ,, Kumar S, and Bresnick EH. (2010) Building Multifunctionality into a Complex Containing Master Regulators of Hematopoiesis. Proc. Natl. Acad. Sci. USA, 107:20429-20434. PMCID: PMC2996669
  • Bresnick EH, Lee HY, Fujiwara T, Johnson KD, and Keles S. (2010) GATA switches as developmental drivers. (minireview) J. Biol. Chem. 285:31087-31093.
  • Snow, JW, Trowbridge JJ, Fujiwara T, Emambokus, N, Grass JA, Orkin SH, and Bresnick EH. (2010) A single cis-element mediates repression of the key developmental regulator GATA-2. PLOS Genet., 9:6(9). PMID: 20838598.