Faculty: Emery H. Bresnick
| Dept: | Professor, Cell & Regenerative Biology |
|---|---|
| Contact: | 4009 WIMR, L4 608-265-6446 ehbresni@wisc.edu |
| Training Areas: | Molecular & Env Toxicology Biostatistics (Affiliate Trainer) Molecular & Cellular Pharmacology Cell & Molecular Biology Biomolecular Chemistry Cancer Biology Biotechnology Pathology & Laboratory Medicine Hematology MD/PhD Translational Cardiovasc Science |
Research Interests
Figure 1
We use multidisciplinary approaches to understand stem/progenitor cell function, normal and malignant blood cell development, and vascular biology. Such approaches include genomics, proteomics, chemical genetics, embryonic stem cell differentiation, computational analysis, and traditional methodologies. In addition to elucidating biological principles, we aim to develop innovative therapeutic strategies based on targeting novel mechanisms.
Mechanisms of hematopoiesis:
We are analyzing the function/regulation of GATA factors that control hematopoietic stem cells (HSCs) and hematopoiesis. Transcriptional profiling and ChIP-seq analysis have identified a cohort of novel GATA factor target genes, including genes encoding proteins that bear no obvious similarity to known proteins. Loss-of-function and gain-of-function studies are being conducted in mice, zebrafish, human adult stem/progenitors, and embryonic stem cell systems to discover new modes of controlling stem/progenitor cell function and to devise novel applications towards translational medicine. Defining novel hematopoietic mechanisms has enormous importance, as deviations from physiological programs yield leukemias, lymphomas, myelodysplasia, and additional blood disorders.
Vascular mechanisms and engineering:
We have developed a novel murine model characterized by severe hematopoietic stem/progenitor cell depletion and a severe vascular integrity defect. We are dissecting mechanisms underlying the establishment and maintenance of vascular integrity in normal and disease states. Understanding such mechanisms is essential for developing efficient approaches to promote the vascularization of engineered tissues, a limiting factor in regenerative medicine.
Go to the Bresnick Lab website.
Honors & Awards
- 2013 - Kellett Mid-Career Award
- 2009 - Editor, The Open Leukemia Journal
- 2008 - Naito Foundation Lecture
- 2007 - Distinguished Alumnus, University of Michigan School of Medicine, Department of Pharmacology
- 2005 - Chair, NIH Erythrocyte and Leukocyte Biology Study Section
- 2005 - Member of Molecular and Cellular Biology Editorial Board
- 2002 - Associate Editor, Journal of Cellular Physiology
- 2002 - Member of Nucleic Acids Research Editorial Board
- 2002 - Member of Journal of Biological Chemistry Editorial Board
- 2002 - Romnes Faculty Scholar
- 2001 - NIH Hematology II Study Section Member
- 2000 - Vilas Associate Award
- 1997 - National Institutes of Health K02 Career Development Award
- 1997 - Leukemia Society of America Scholar Award
- 1996 - Shaw Scientist Award
- 1994 - Pharmaceutical Manufacturers of America Foundation Faculty Development Award
Recent Invited Lectures
- Red Cell Gordon Conference, 2011
- 18th International Hemoglobin Switching Meeting, Asilomar, 2012
- Dana Farber Cancer Institute, Harvard Medical School, 2012
- 5th International GATA factor meeting, Sendai, Japan, 2010
- 17th International Hemoglobin Switching Meeting, Oxford, 2010
- NIH (NIDDK), Laboratory of Cellular and Developmental Biology, 2010
- European Hematology Association, Barcelona, 2010
- British Society of Hematology, Brighton, UK, 2009
- Weatherall Institute of Molecular Medicine, Oxford University, 2009
- Red Cell Gordon Conference, University of New England, 2009
- Naito Foundation Symposium: Nuclear Dynamics and RNA, Japan, 2008
- 16th International Hemoglobin Switching Meeting, Asilomar, CA, 2008
- American Society of Hematology, San Francisco, CA, 2008
- University of Montreal, September, 2007
- Red Cell Gordon Conference, France, 2007
- Loyola University, Cardinal Bernardin Cancer Center, 2007
- 4th International GATA factor meeting, Capri, Italy, 2007
- Johns Hopkins University School of Medicine, Department of Pharmacology and Molecular Sciences, 2007
- Yale University, Department of Genetics, 2006
- 15th International Hemoglobin Switching Meeting, Oxford, 2006
- Yale University, Center for Molecular Hematology, 2006
- Kansas University School of Medicine, 2006
Recent Study Section Service
- Chair, NIH Stem Cell Therapeutics II Special Emphasis Panel, 2012
- Chair, NIH Erythrocyte Biology Special Emphasis Panel, 2010
- American Heart Association Basic Cell and Molecular Biology 4 Study Section, 2009
- Chair, NIH Erythrocyte and Leukocyte Biology Study Section, 2005-2007
- Member of NIH Study Section Erythrocyte and Leukocyte Biology, 2003-2007
Other Links
Selected Publications (Find recent publications on PubMed)
- Pope NJ, Bresnick EH. Establishment of a Cell Type-Specific Genetic Network by the Mediator Complex Component Med1. Mol Cell Biol. 2013 Mar 4 [Epub ahead of print] PMID: 23459945
- Guiu J, Shimizu R, D'Altri T, Fraser ST, Hatakeyama J, Bresnick EH, Kageyama R, Dzierzak E, Yamamoto M, Espinosa L, Bigas A. Hes repressors are essential regulators of hematopoietic stem cell development downstream of Notch signaling. J Exp Med. 2013 Jan 14;210(1):71-84. doi: 10.1084/jem.20120993. Epub 2012 Dec 24. PMID 23267012
- Fujiwara T, Yokoyama H. Okitsu Y, Kamata M, Fukuhara N, Onishi Y, Fujimaki S, Takahashi S, Ishizawa K, Bresnick EH, Harigae H. Gene expression profiling identifies HOXB4 as a direct downstream target of GATA-2 in human CD34+ hematopoietic cells. PLoS One. 2012;7(9):e40959. doi: 10.1371/journal.pone.0040959. Epub 2012 Sep 24. PMID: 23028422
- Johnson KD, Hsu AP, Ryu M-J, Wang J, Gao X, Boyer ME, Keles S, Lee ., Calvo KR, Zhang J, Holland SM, and Bresnick EH. (2012) Cis-element mutated in GATA2-dependent immunodeficiency governs hematopoiesis and vascular integrity. J. Clin. Invest. 122:3692-3704. PMCID: PMC3461907
- Kang YA, Sanalkumar R, O'Geen H, Linnemann AK, Chang CJ, Bouhassira EE, Farnham PJ, Keles S, Bresnick EH (2012). Autophagy driven by a master regulator of hematopoiesis. Mol Cell Biol. 32:226-239. PMID: 22025678
- Linnemann AK, O'Geen H, Keles S, Farnham PJ, Bresnick EH (2011). Genetic framework for GATA factor function in vascular biology. Proc Natl Acad Sci U S A. 108:13641-13646. PMID: 21808000
- Chung D, Kuan PF, Li B, Sanalkumar R, Liang K, Bresnick EH, Dewey C, Kele_ S (2011). Discovering transcription factor binding sites in highly repetitive regions of genomes with multi-read analysis of ChIP-Seq data. PLoS Comput Biol. 7(7):e1002111. PMID: 21779159
- Lee HY, Johnson KD, Boyer ME, Bresnick EH (2011). Relocalizing genetic loci into specific subnuclear neighborhoods. J Biol Chem. 286:18834-18844. PMID: 21398517
- Fujiwara T, Lee HY, Sanalkumar R, Bresnick EH (2010). Building multifunctionality into a complex containing master regulators of hematopoiesis. Proc Natl Acad Sci U S A.107:20429-20434. PMID: 21059912
- Bresnick EH, Lee HY, Fujiwara T, Johnson KD, and Keles S (2010). GATA switches as developmental drivers. J Biol Chem. 285:31087-93. PMID: 20670937
- Pope NJ, Bresnick EH (2010). Differential coregulator requirements for function of the hematopoietic transcription factor GATA-1 at endogenous loci. Nucleic Acids Res. 38:2190-2200. PMID: 20047963
- Fujiwara T, Lee HJ,, Kumar S, and Bresnick EH. (2010) Building Multifunctionality into a Complex Containing Master Regulators of Hematopoiesis. Proc. Natl. Acad. Sci. USA, 107:20429-20434. PMCID: PMC2996669
- Bresnick EH, Lee HY, Fujiwara T, Johnson KD, and Keles S. (2010) GATA switches as developmental drivers. (minireview) J. Biol. Chem. 285:31087-31093.
- Snow, JW, Trowbridge JJ, Fujiwara T, Emambokus, N, Grass JA, Orkin SH, and Bresnick EH. (2010) A single cis-element mediates repression of the key developmental regulator GATA-2. PLOS Genet., 9;6(9). PMID: 20838598.