Faculty: Ron Raines
| Dept: | Professor, Biochemistry & Chemistry |
|---|---|
| Contact: | 371B Biochem 608-262-8588 raines@biochem.wisc.edu |
| Training Areas: | Molecular & Cellular Pharmacology Cellular & Molecular Biology Integrated Program in Biochemistry Chemistry Biophysics Cancer Biology MSTP |
Research Interests
The amino acid sequence of a protein encodes its three-dimensional structure, and this structure manifests itself in biological function. Using techniques that range from synthetic chemistry to biochemistry to cell biology, the Raines group is illuminating in atomic detail both the chemical basis and the biological purpose for protein structure and protein function. The efforts of the Raines group are leading to insights into the relationship between amino acid sequence and protein function (or dysfunction), as well as to the creation of novel molecules with desirable properties. The research projects in the Raines laboratory are designed to reveal how biological phenomena can be explained with the principles of chemistry. The hypotheses are far-reaching, and testing them requires the use of techniques and ideas from diverse disciplines. This broad/deep training is appropriate for scientists who want to perform innovative and meaningful research at the widening chemistry - biology interface.
Honors & Awards
- 2010 - Repligen Award, American Chemical Society
- 2008 - Welch Lecturer, Welch Foundation
- 2007 - Rao Makineni Lectureship Award, American Peptide Society
- 2006 - RSC Fellow, Royal Society of Chemistry (London)
- 2005 - Emil Thomas Kaiser Award, Protein Society
- 2004 - Arthur C. Cope Scholar Award, American Chemical Society
- 2002 - AAAS Fellow, American Association for the Advancement of Science
- 2001-02 - Guggenheim Fellow, J. S. Guggenheim Memorial Foundation
- 1998 - Pfizer Award, American Chemical Society
Selected Publications
- Levine MN and Raines RT (2012). Trimethyl lock: A trigger for molecular release in chemistry, biology, and pharmacology. Chem Sci. 3:2412-2420.
- Lomax JE, Eller CH, Raines RT (2012). Rational design and evaluation of Mammalian ribonuclease cytotoxins. Methods Enzymol. 502:273-290. PMID: 22208989
- Choudhary A, Kamer KJ, Raines RT (2012).A conserved interaction with the chromophore of fluorescent proteins. Protein Sci. 21:171-7. doi: 10.1002/pro.762. PMID: 22057893
- Ellis GA, McGrath NA, Palte MJ, Raines RT (2012). Ribonuclease-activated cancer prodrug. ACS Med Chem Lett 3:268-272.
- Palte MJ and Raines RT (2012). Interaction of nucleic acids with the glycocalyx. J Am Chem Soc 134:6218-6223.
- Ellis GA, Palte MJ, Raines RT (2012). Boronate-mediated biologic delivery. J Am Chem Soc 134:3631-3634.
- Sundlass NK and Raines RT (2011). Arginine residues are more effective than lysine residues in eliciting the cellular uptake of a cytotoxic protein. Biochemistry. 50:10293-10299.
- Smith BD, Higgin JJ, Raines RT (2011). Site-specific folate conjugation to a cytotoxic protein. Bioorg Med Chem Lett. 21:5029-5032.
- Rutkoski TJ, Kink JA, Strong LE, and Raines RT (2011). Site-specific PEGylation endows a mammalian ribonuclease with antitumor activity. Cancer Biol Ther. 12:208-214.
- Ellis GA, Hornung ML, and Raines RT (2011). Potentiation of ribonuclease cytotoxicity by a poly(amidoamine) dendrimer. Bioorg Med Chem Lett. 21:2756-2758.