Faculty: David Beebe
| Dept: | Professor, Biomedical Engineering |
|---|---|
| Contact: | 6009 Wisconsin Institutes for Medical Research 1111 Highland Ave Madison, WI (608) 262-2260 djbeebe@wisc.eu |
| Training Areas: | Biotechnology, Genomic Sciences, Stem Cell, Chemistry and Biology, Molecular and Cellular Pharmacology Program |
| Lab Page: | Click Here |
Research Description
Basic cell culture techniques have changed little in almost a century. By far the most dominate format for cell culture is the Petri dish or similar (e.g. multi well plate). Slowly this is beginning to change as our ability to create materials, geometries and even systems at the cellular scale continues to grow and mature. At the same time, the importance of the three-dimensional microenvironment in cancer biology is increasingly demonstrated. We have begun to explore a variety of engineered in vitro microenvironments to probe the nature of cell interactions that regulate cell behavior. These environments range from in silico lineage models to three-dimensional co-culture constructs to simple convective-free culture systems to high throughput assay systems. While our particular interests center around cancer biology, the constructs have broad potential application across cell biology. Our goal is a holistic approach to understanding cell behavior that integrates in vitro cellular scale engineering to recapitulate important in vivo microenvironmental characteristics in ways that provide biological insights, aid in diagnosis/treatment and enhance discovery.
Honors & Awards
- Founding Scientific Editor, Integrative Biology, Royal Society of Chemistry, 2009
- Fellow, American Institute for Medical and Biological Engineering (AIMBE), 2007
- Lab on a Chip, Royal Society of Chemistry and Corning, Pioneers of Miniaturization Prize, 2006
- Fellow, Royal Society of Chemistry, 2005
- Romnes Award, UW-Madison, 2003
- IEEE EMBS Early Career Achievement Award, 2001
Selected Publications
(Find further recent publications on PubMed)
- Warrick JW, Young EW, Schmuck EG, Saupe KW, Beebe DJ. High-content adhesion assay to address limited cell samples. Integr Biol (Camb). 2013 Feb 21. [Epub ahead of print] PMID: 23426645
- Montanez-Sauri SI, Sung KE, Berthier E, Beebe DJ. Enabling screening in 3D microenvironments: probing matrix and stromal effects on the morphology and proliferation of T47D breast carcinoma cells. Integr Biol (Camb). 2013 Feb 25;5(3): 631-40. doi: 10.1039/c3ib20225a. PMID: 23340769
- Su X, Theberge AB, January CT, Beebe DJ. Effect of microculture on cell metabolism and biochemistry: do cells get stressed in microchannels? Anal Chem. 2913 Feb 5;85(3):1562-70. doi: 10.1021/ac3027228. Epub 2013 Jan 17. PMID: 23327437
- Young EW, Berthier E, Beebe DJ. Assessment of enhanced autofluorescence and impact on cell microscopy for microfabricated thermoplastic devices. Anal Chem 2013 Jan 2;85;(1):44-9. doi: 10.1021/ac3034773. Epub 2012 Dec 18. PMID: 23249264
- Berthier E, Guckenberger DJ, Cavnar P, Huttenlocher A, Keller NP, Beebe DJ. Kit-On-A-Lid-Assays for accessible self-contained cell assays. Lab Chip. 2013 Feb 7;13(3):424-31. doi: 10.1039/c2lc41019b. PMID: 23229806
- Casavant BP, Guckenberger DJ, Berry SM, Tokar JT, Lang JM, Beebe DJ. The VerIFAST: an integrated method for cell isolation and extracellular/intracellular staining. Lab Chip 2013 Feb 7;13(3):391-6. doi: 10.1039/c2lc41136a. PMID: 23223939
- Bischel LL, Young EW, Madar BR, Beebe DJ. Tubeless microfluidic angiogenesis assay with three-dimensional endothelial-lined microvessels. Biomaterials. 2013 Feb;34(5):1471-7. doi: 10.1016/j.biomaterials.2012.11.005. Epub 2012 Nov 26. PMID: 23191982
- Su G, Sung KE, Beebe DJ, Friedl A. Functional screen of paracrine signals in breast carcinoma fibroblasts. PLoS One. 2012;7(10):e46685. doi: 10.1371/journal.pone.0046685. Epub 2012 Oct 8. PMID: 23056402
- Berry SM, Regehr KJ, Casavant BP, Beebe DJ. Automated Operation of Immiscible Filtration Assisted by Surface Tension (IFAST) Arrays for Streamlined Analyte Isolation. J Lab Autom. 2012 Sep 26. [Epub ahead of print] PMID: 23015519
- Sackmann EK, Berthier E, Young EW, Shelef MA, Wernimont SA, Huttenlocher A, Beebe DJ. Microfulidic kit-on-a-lid: a versatile platform for neutrophil chemotaxis assays. Blood. 2012 Oct 4;120(14):e45-53. doi: 10.1182/blood-2012-03-416453. Epub 2012 Aug 22. PMID: 22915642
- Strotman LN, Lin G, Berry SM, Johnson EA, and Beebe DJ. (2012) Facile and rapid DNA extraction and purification from food matrices using IFAST (immiscible filtration assisted by surface tension). Analyst. 2012 Jul 20. [Epub ahead of print] PMID: 22814365
- Lang JM, Casavant BP, and Beebe DJ. (2012) Circulating tumor cells: getting more from less. Sci Transl Med. 4:141ps13.PMID: 22764205
- Cavnar PJ, Mogen K, Berthier E, Beebe DJ, Huttenlocher A. (2012) The Actin Regulatory Protein HS1 Interacts with Arp2/3 and Mediates Efficient Neutrophil Chemotaxis. J Biol Chem. 287:25466-25477. Epub 2012 Jun 7. PMID: 22679023
- Berry SM, Maccoux LJ, and Beebe DJ. (2012) Streamlining immunoassays with immiscible filtrations assisted by surface tension. Anal Chem. 84:5518-5523. Epub 2012 Jun 19.PMID: 22632629
- Guckenberger DJ, Berthier E, Young EW, and Beebe DJ. (2012) Induced hydrophobic recovery of oxygen plasma-treated surfaces. Lab Chip. 12:2317-2321. PMID: 22592853
- Resto PJ, Berthier E, Beebe DJ, and Williams JC. (2012) An inertia enhanced passive pumping mechanism for fluid flow in microfluidic devices. Lab Chip. 12:2221-2228. PMID: 22441561
- Goel S, Chin EN, Fakhraldeen SA, Berry SM, Beebe DJ, and Alexander CM. (2012) Both LRP5 and LRP6 receptors are required to respond to physiological Wnt ligands in mammary epithelial cells and fibroblasts. J Biol Chem. 287:16454-16466.