Hogune Im, PhD

Graduation: 02/2006
Advisor: Bresnick, Emery
Current Position:

Senior Genomics Scientist
Samsung SDS
Songpa-gu, Seoul, South Korea

Research Interest: Investigating the cause of human disease using combination of next-generation sequencing technology and iPS cell, investigating global methyltransferase-substrate network using proteomics approaches, protein microarray, mass spectrometry
Email: hoguneim (at) stanford (dot) edu

Alumni News

  • Current Research: NGS projects involving ultra-low and single cell RNA-seq to identify disease/therapeutic target of human diseases (MDS, IPAH, Type I diabetes).
    Quantitative proteomics in human samples to monitor quantitative changes.
    Integration of proteome and genomic data to predict phenotypic outcomes and derive hypothesis about mechanisms of regulation
    Personal interest in transcriptional mechanisms and signaling pathways that regulate chromatin structures by epistatic mechanism in establishing tissue specificity during developmental.
  • Specialties – high-throughput cloning, expression and purification in various systems;plant, yeast, insect cells, mammalian cells.
    chromatin IP, Protein expression/isolation, Molecular biology, Epigenetics, Biochemistry, Developmental Biology
  • Stanford University – October 2011 – Present (1 year 1 month) San Francisco Bay Area
  • Skills: Molecular Biology, Molecular Cloning, Protein Expression, Biochemistry, qPCR, Cells
  • Interested in transcriptional mechanisms and signaling pathways that regulate chromatin structures.
  • Previously a Postdoc at Yale University for 3 years.

Publications

  • Im H and Snyder M (2013). Preparation of recombinant protein spotted arrays for proteome-wide identification of kinase targets. Curr Protoc Protein Sci. April 2013. PMID:23546622
  • Im H and Snyder M (2013). Identification of Potential Glycan Cancer Markers with Sialic Acid Attached to Sialic Acid and Up-regulated Fucosylated Galactose Structures in Epidermal Growth Factor Receptor Secreted from A431 Cell LineMol Cell Proteomics.12:1239-1249. PMID:23371026. First Published on January 31, 2013
  • Liu S, Im H, Bairoch A, Cristofanilli M, and et al. (2013). A chromosome-centric human proteome project (C-HPP) to characterize the sets of proteins encoded in chromosome 17. J Proteome Res. 12:45-57. PMIC:23259914
  • Chen R, Mias GI, Li-Pook-Than J, Jiang L, Lam HY, Chen R, Miriami E, Karczewski KJ, Hariharan M, Dewey FE, Cheng Y, Clark MJ, and Im H et al. (2012). Personal omics profiling reveals dynamic molecular and medical phenotypes. Cell. 48:1293-1307. PMID: 22424236
  • Mok J, Im H, and Snyder M (2009). Global identification of protein kinase substrates by protein microarray analysis. Nat Protoc. 4:1820-1827. Epub. PMCID: PMC3382020
  • Sawado T, Halow J, Im H, Ragoczy T, Bresnick EH, Bender MA, and Groudine M (2008). H3 K79 dimethylation marks developmental activation of the beta-globin gene but is reduced upon LCR-mediated high-level transcription. Blood.112:406-414. PMCID: PMC2442750
  • Bresnick EH, Im H, Johnson KD, and Kim SI (2006). Long-range transcriptional activation: Studies with the b-globin system. Establishment and Regulation of Chromaitn Domains: Mechanistic Insights from Studies of Hemoglobin Synthesis.  Prog Nuc Res Mol Biol. 81:435-471. PMID:16891178
  • Im H, Grass JA, Johnson KD, Kim SI, Boyer ME, Imbalzano AN, Bieker JJ, and Bresnick EH (2005). Chromatin domain activation via GATA-1 utilitzation of a small subset of dispersed GATA motifs within a broad chromosomal region. PNAS USA.102:17065-17070. PDF PMCID: PMC1287986
  • Im H*, Grass JA* Johnson KD, Boyer ME, Wu J, and Bresnick EH (2004).Measurement of protein-DNA interactions in vivo by chromatin immunoprecipitation. Methods Mol Biol:  Signal Transduction Protocols. 284:129-146. (*co-first authors) PMID:15173613
  • Johnson KD, Grass JA, Park C, Im H, Choi K, and Bresnick EH (2003). Highly restricted localization of RNA polymerase II within a locus control region of a tissue-specific chromatin domain. Mol Cell Biol. 23:6488-6493. PDF PMCID: PMC193707
  • Bresnick EH, Im H, and Johnson KD (2003). Histone acetylation: long range patterns in the genome. Encyclopedia of the Human Genome. Nature Publishing Company. 260-264. 1st edition.
  • Im H, Park C, Feng Q, Johnson KD, Kiekhaefer CM, Choi K, Zhang Y, and Bresnick EH (2003). Dynamic regulation of histone H3 methylated at lysine 79 within a tissue-specific chromatin domain. J Biol Chem. 278:18346-18352. PDFPMID:12604594
  • Im H, Grass JA, Christensen HM, Perkins A, and Bresnick EH (2002). Histone deacetylase-dependent establishment and maintenance of broad low-level histone acetylation within a tissue-specific chromatin domain. Biochem.41:15152-15160. PDF PMID:12484752
  • Khattak S, Im H, Park T, Ahnn J, and Spoerel NA (2002). dELL, a drosophila homologue of transcription elongation factor ELL (Eleven-nineteen Lysine rich Leuke) is required for early development. Cell Biochem Funct. 20:119-127. PMID:11979508
  • Forsberg EC, Downs KM, Christensen HM, Im H, Nuzzi PA, and Bresnick EH (2000). Developmentally dynamic histone acetylation pattern of a tissue-specific chromatin domain. PNAS USA. 97:14494-14499. PDF PMCID: PMC18947