Our lab focuses on germline stem cells (GSCs) and their regulation in the nematode C. elegans. GSCs reside within a stem cell niche and generate daughter cells that differentiate (see figure). This niche uses Notch signaling to maintain GSCs. Notch signaling in turn activates a regulatory network to maintain germ cells in a stem cell state or to drive them into differentiation. FBF, a conserved sequence-specific RNA binding protein, provides the major hub for maintenance of adult stem cells. FBF functions as a broad-spectrum regulator of differentiation with many target mRNAs shared with its human PUF counterparts. We continue to analyze regulators of stem cell maintenance, regulators of differentiation as sperm or oocyte and the molecular network that encompasses both stem maintenance and differentiation.
Honors & Awards
- President, Society for Developmental Biology, 2004-2005
- Hilldale Award, Biological Sciences Division, 2003
- American Philosophical Society, 2002
- Vilas Professorship, 2001
- National Academy of Sciences, 1elected in 1995
- Byrd DT, Knobel K, Affeldt K, Crittenden SL,Kimble J. (2014) A DTC niche plexus surrounds the germline stem cell pool in Caenorhabditis elegans. PLoS One. 9(2):e88372. eCollection 2014. PMID: 24586318 [PubMed – in process]
- Kershner, A.M., Shin, H., Hansen, T.J. and J. Kimble (2014) Discovery of two GLP-1/Notch targetgenes that account for the role of GLP-1/Notch signaling in stem cell maintenance. PNAS111(10), 3739-3744
- Morgan CT, Noble D, and Kimble J (2013). Mitosis-meiosis and sperm-oocyte fate decisions are separable regulatory events. Proc Natl Acad Sci U S A. 110:3411-6. Epub 2013 Feb 11. PMID: 23401507
- Friend K, Campbell ZT, Cooke A, Kroll-Conner P, Wickens MP and Kimble J(2012). A conserved PUF/Ago/eEF1A complex attenuates translation elongation. Nature Structural and Molecular Biology 19:176-183.
- Cinquin O, Crittenden SL, Morgan DE, and Kimble J (2010). Progression from a stem cell-like state to early differentiation in the C. elegans germ line. PNAS 107:2048-2053.
- Morgan CT, Lee M-H, and Kimble J (2010). Chemical reprogramming of Caenorhabditis elegans germ cell fate. Nature Chemical Biology 6:102-104.