University of Wisconsin–Madison

Emery Bresnick, Phd

Professor, Cell & Regenerative Biology; Director, UW Madison Blood Research Program

ehbresni@wisc.edu

608-262-6446

4009 WIMR, L4
1111 Highland Ave

Lab Website
UW-Madison Blood Research Program

Emery Bresnick

Research Interests

We use multidisciplinary approaches to understand stem/progenitor cell function, normal and malignant blood cell development, and vascular biology. Such approaches include genomics, proteomics, chemical genetics, embryonic stem cell differentiation, computational analysis, and traditional methodologies. In addition to elucidating biological principles, we aim to develop innovative therapeutic strategies based on targeting novel mechanisms.

Mechanisms of hematopoiesis:

We are analyzing the function/regulation of GATA factors that control hematopoietic stem cells (HSCs) and hematopoiesis. Transcriptional profiling and ChIP-seq analysis have identified a cohort of novel GATA factor target genes, including genes encoding proteins that bear no obvious similarity to known proteins. Loss-of-function and gain-of-function studies are being conducted in mice, zebrafish, human adult stem/progenitors, and embryonic stem cell systems to discover new modes of controlling stem/progenitor cell function and to devise novel applications towards translational medicine. Defining novel hematopoietic mechanisms has enormous importance, as deviations from physiological programs yield leukemias, lymphomas, myelodysplasia, and additional blood disorders.

Vascular mechanisms and engineering:

We have developed a novel murine model characterized by severe hematopoietic stem/progenitor cell depletion and a severe vascular integrity defect. We are dissecting mechanisms underlying the establishment and maintenance of vascular integrity in normal and disease states. Understanding such mechanisms is essential for developing efficient approaches to promote the vascularization of engineered tissues, a limiting factor in regenerative medicine.

Honors & Awards

  • 2013 – Kellett Mid-Career Award
  • 2009 – Editor, The Open Leukemia Journal
  • 2008 – Naito Foundation Lecture
  • 2007 – Distinguished Alumnus, University of Michigan School of Medicine, Department of Pharmacology
  • 2005 – Chair, NIH Erythrocyte and Leukocyte Biology Study Section
  • 2005 – Member of Molecular and Cellular Biology Editorial Board

Recent Invited Lectures

  • Red Cell Gordon Conference, 2013
  • International Society of Experimental Hematology and Stem Cell Biology, 2013
  • Mt. Sinai School of Medicine, 2013
  • University of Illinois-Chicago, 2013
  • 18th International Hemoglobin Switching Meeting, Asilomar, 2012
  • Dana Farber Cancer Institute, Harvard Medical School, 2012
  • Red Cell Gordon Conference, 2011
  • 5th International GATA factor meeting, Sendai, Japan, 2010
  • 17th International Hemoglobin Switching Meeting, Oxford, 2010
  • NIH (NIDDK), Laboratory of Cellular and Developmental Biology, 2010
  • European Hematology Association, Barcelona, 2010

Recent Study Section Service

  • Chair, NIH Stem Cell Therapeutics II Special Emphasis Panel, 2012
  • Chair, NIH Erythrocyte Biology Special Emphasis Panel, 2010
  • American Heart Association Basic Cell and Molecular Biology 4 Study Section, 2009
  • Chair, NIH Erythrocyte and Leukocyte Biology Study Section, 2005-2007
  • Member of NIH Study Section Erythrocyte and Leukocyte Biology, 2003-2007

Other Links

Selected Publications

(Find further publications on PubMed)

  • Hewitt KJ, Johnson KD, Gao X, Keles S, Bresnick EH. The Hematopoietic Stem and
    Progenitor Cell Cistrome: GATA Factor-Dependent cis-Regulatory Mechanisms. Curr
    Top Dev Biol. 2016;118:45-76. doi: 10.1016/bs.ctdb.2016.01.002. Epub 2016 Feb 26.
    PubMed PMID: 27137654.
  • Gao X, Wu T, Johnson KD, Lahvic JL, Ranheim EA, Zon LI, Bresnick EH. GATA
    Factor-G-Protein-Coupled Receptor Circuit Suppresses Hematopoiesis. Stem Cell
    Reports. 2016 Mar 8;6(3):368-82. doi: 10.1016/j.stemcr.2016.01.008. Epub 2016 Feb
    18. PubMed PMID: 26905203; PubMed Central PMCID: PMC4788764.
  • Tanimura N, Miller E, Igarashi K, Yang D, Burstyn JN, Dewey CN, Bresnick EH.
    Mechanism governing heme synthesis reveals a GATA factor/heme circuit that
    controls differentiation. EMBO Rep. 2016 Feb;17(2):249-65. doi:
    10.15252/embr.201541465. Epub 2015 Dec 23. PubMed PMID: 26698166.
  • Johnson KD, Kong G, Gao X, Chang YI, Hewitt KJ, Sanalkumar R, Prathibha R,
    Ranheim EA, Dewey CN, Zhang J, Bresnick EH. Cis-regulatory mechanisms governing
    stem and progenitor cell transitions. Sci Adv. 2015 Sep 4;1(8):e1500503. doi:
    10.1126/sciadv.1500503. eCollection 2015 Sep. PubMed PMID: 26601269; PubMed
    Central PMCID: PMC4643771.
  • Hewitt KJ, Kim DH, Devadas P, Prathibha R, Zuo C, Sanalkumar R, Johnson KD,
    Kang YA, Kim JS, Dewey CN, Keles S, Bresnick EH. Hematopoietic Signaling
    Mechanism Revealed from a Stem/Progenitor Cell Cistrome. Mol Cell. 2015 Jul
    2;59(1):62-74. doi: 10.1016/j.molcel.2015.05.020. Epub 2015 Jun 11. PubMed PMID:
    26073540; PubMed Central PMCID: PMC4499333.
  • DeVilbiss AW, Sanalkumar R, Hall BD, Katsumura KR, de Andrade IF, Bresnick EH.
    Epigenetic Determinants of Erythropoiesis: Role of the Histone Methyltransferase
    SetD8 in Promoting Erythroid Cell Maturation and Survival. Mol Cell Biol. 2015
    Jun;35(12):2073-87. doi: 10.1128/MCB.01422-14. Epub 2015 Apr 8. PubMed PMID:
    25855754; PubMed Central PMCID: PMC4438249.
  • McIver SC, Kang YA, DeVilbiss AW, O’Driscoll CA, Ouellette JN, Pope NJ,
    Camprecios G, Chang CJ, Yang D, Bouhassira EE, Ghaffari S, Bresnick EH. The
    exosome complex establishes a barricade to erythroid maturation. Blood. 2014 Oct
    2;124(14):2285-97. doi: 10.1182/blood-2014-04-571083. Epub 2014 Aug 12. PubMed
  • PMID: 25115889; PubMed Central PMCID: PMC4183988.
    Katsumura KR, Yang C, Boyer ME, Li L, Bresnick EH. Molecular basis of
    crosstalk between oncogenic Ras and the master regulator of hematopoiesis GATA-2.
    EMBO Rep. 2014 Sep;15(9):938-47. doi: 10.15252/embr.201438808. Epub 2014 Jul 23.
    PubMed PMID: 25056917; PubMed Central PMCID: PMC4198037.
  • Yamazaki H, Suzuki M, Otsuki A, Shimizu R, Bresnick EH, Engel JD, Yamamoto M.
    A remote GATA2 hematopoietic enhancer drives leukemogenesis in inv(3)(q21;q26) by
    activating EVI1 expression. Cancer Cell. 2014 Apr 14;25(4):415-27. doi:
    10.1016/j.ccr.2014.02.008. Epub 2014 Apr 3. PubMed PMID: 24703906; PubMed Central
    PMCID: PMC4012341.
  • Gao X, Johnson KD, Chang YI, Boyer ME, Dewey CN, Zhang J, Bresnick EH. Gata2
    cis-element is required for hematopoietic stem cell generation in the mammalian
    embryo. J Exp Med. 2013 Dec 16;210(13):2833-42. doi: 10.1084/jem.20130733. Epub
    2013 Dec 2. PubMed PMID: 24297994; PubMed Central PMCID: PMC3865483.