Suzanne Ponik, PhD
Position title: Senior Scientist, Assistant Faculty Associate, Department of Cell and Regenerative Biology
1111 Highland Ave
- Lab Website
- Ponik Lab
My group is interested in understanding the molecular mechanisms underlying breast cancer risk due to breast density. Patients with mammographically dense breast tissue have a four to six-fold increased risk of developing breast carcinomas. In fact, 1/3 of all breast cancer cases are attributed to breast density, making it one of the greatest risk factors for carcinoma. Increased breast density is associated with a significant increase in the deposition of extracellular matrix (ECM) components, most notably the protein, collagen. We have developed in vitro and in vivo model systems to understand why increased breast density results in an increased risk for developing breast carcinoma. Additionally, our group uses multiphoton microscopy and intravital imaging approaches to characterize the collagen structure surrounding tumors so that we can better understand the physical relationship between cells and the collagen fibers found in breast tissue.
We are particularly interested in molecular signaling events related to cell interactions with the ECM. During oncogenic transformation, normal interactions with the ECM are profoundly altered, resulting in cells that lose their polarization and differentiation, lose anchorage dependent growth control, and acquire a migratory, invasive phenotype. Further, we have identified an overall increase in inflammatory signals in tumors that arise in a collagen dense microenvironment. Together, these data demonstrate that collagen density creates an invasive, inflammatory tumor microenvironment leading to enhanced disease progression. We are interested in understanding how physical changes in the ECM determine cell phenotype and signaling related to the recruitment, differentiation and polarization of cells in the tumor microenvironment.
- Hoffmann EJ, Ponik SM. Biomechanical Contributions to Macrophage Activation in the Tumor Microenvironment. Front Oncol. 2020;10:787. doi: 10.3389/fonc.2020.00787. eCollection 2020. Review. PubMed Central PMCID: PMC7251173.
- Lugo-Cintrón KM, Gong MM, Ayuso JM, Tomko LA, Beebe DJ, Virumbrales-Muñoz M, Ponik SM. Breast Fibroblasts and ECM Components Modulate Breast Cancer Cell Migration Through the Secretion of MMPs in a 3D Microfluidic Co-Culture Model. Cancers (Basel). 2020 May 6;12(5). doi: 10.3390/cancers12051173. PubMed Central PMCID: PMC7281408.
- Sung BH, von Lersner A, Guerrero J, Krystofiak ES, Inman D, Pelletier R, Zijlstra A, Ponik SM, Weaver AM. A live cell reporter of exosome secretion and uptake reveals pathfinding behavior of migrating cells. Nat Commun. 2020 Apr 29;11(1):2092. doi: 10.1038/s41467-020-15747-2. PMCID: PMC7190671.
- Jallow F, O’Leary KA, Rugowski DE, Guerrero JF, Ponik SM, Schuler LA. Dynamic interactions between the extracellular matrix and estrogen activity in progression of ER+ breast cancer. Oncogene. 2019 Oct;38(43):6913-6925. doi: 10.1038/s41388-019-0941-0. PubMed Central PMCID: PMC6814534.
- Tomko,LA, Hill RC, Barrett A, Szulczewski JM, Conklin MW, Eliceiri KW, Keely PJ, Hansen KC, Ponik SM.Targeted matrisome analysis identifies thrombospondin-2 and tenascin-C in aligned collagen stroma from invasive breast carcinoma. Sci Rep.2018 Aug 28;8(1):12941 PMCID: PMC6113240
- Esbona K, Yi Y, Saha S, Yu M, Van Doorn RR, Conklin MW, Graham DS, Wisinski KB, Ponik SM, Eliceiri KW, Wilke LG, Keely PJ. The presence of COX-2, tumor-associated macrophages, and collagen alignment as prognostic markers for invasive breast carcinoma patients.Am J Pathol. 2018 Mar;188(3):559-573 PMCID:PMC5963475