Gail Seabold, PhD
Position title: Scientific Skills Coach, National Institutes of Health, Washington D.C. Area
Email: gail.seabold@nih.com
Phone: Advisor: Dr. Johannes Hell
Address:
Graduation: 08/2003
Alumni News
- Our lab has identified a new family of adhesion molecules that we have called Synaptic Adhesion-Like Molecules (SALMs). This family of proteins contains domains similar to those found in proteins previously reported to be involved in cell adhesion, neurite outgrowth, and synaptic plasticity. We found that SALMs are expressed at an early stage in the brain and that their expression continues to increase as the brain develops. The identification of this novel family of adhesion-like molecules that may play a role in neurite outgrowth and synapse development is an interesting finding. However, one intriguing aspect of this family of proteins is their association with NMDA receptors.The association of SALMs with the NMDA receptor was observed by immunoprecipitating the SALMs from rat brain and probing with antibodies to the NMDA receptor. We investigated this further by showing that over-expressing SALM proteins led to a co-clustering of SALM with the NMDA receptor. This interaction appears to occur through the members of the PSD-95 protein family since both SALMs and the NMDA receptor interact with these proteins. However, our studies also revealed a direct interaction between the NMDA receptor and SALMs, which occurs through the extracellular part of these proteins. How this interaction occurs and its physiological significance are currently being investigated, but one possible role is that this interaction stabilizes one or both of these molecules on the cell surface.
- 2006: Received the Fellows Award for Research Excellence, NIH, Bethesda, MD.
Publications
- Seabold GK, Wang PY, Petralia RS, Chang K, Zhou A, McDermott MI, Wang YX, Milgram SL, and Wenthold RJ (2012). Dileucine and PDZ-binding motifs mediate synaptic adhesion-like molecule 1 (SALM1) trafficking in hippocampal neurons. J Biol Chem. 287:4470-4484. PMCID: PMC3281672
- Cuzon Carlson VC, Seabold GK, Helms CM, Garg N, Odagiri M, Rau AR, Daunais J, Alvarez VA, Lovinger DM, and Grant KA (2011). Synaptic and morphological neuroadaptations in the putamen associated with long-term, relapsing alcohol drinking in primates. Neuropsychopharmacology. 36:2513-2528. PMCID: PMC3194078. [PubMed – in process]
- Zheng CY, Seabold GK, Horak M, and Petralia RS (2011). MAGUKs, synaptic development, and synaptic plasticity. Neuroscientist. 17:493-512. PMCID: PMC3191319. [PubMed – indexed for MEDLINE]
- Dobi A, Seabold GK, Christensen CH, Bock R, and Alvarez VA (2011).Cocaine-induced plasticity in the nucleus accumbens is cell specific and develops without prolonged withdrawal. J Neurosci. 31:1895-904. PMCID: PMC3040105. [PubMed – indexed for MEDLINE]
- Seabold GK, Daunais JB, Rau A, Grant KA, and Alvarez VA (2010). DiOLISTIC labeling of neurons from rodent and non-human primate brain slices. J Vis Exp. (41). PMCID: PMC3156079
- Brigman JL, Wright T, Talani G, Prasad-Mulcare S, Jinde S, Seabold GK, Mathur P, Davis MI, Bock R, Gustin RM, Colbran RJ, Alvarez VA, Nakazawa K, Delpire E, Lovinger DM, and Holmes A (2010). Loss of GluN2B-containing NMDA receptors in CA1 hippocampus and cortex impairs long-term depression, reduces dendritic spine density, and disrupts learning. J Neurosci. 30:4590-600. PMCID: PMC2869199
- Chang K, Seabold GK, Wang CY, and Wenthold RJ (2010). Reticulon 3 is an interacting partner of the SALM family of adhesion molecules. J Neurosci Res.88:266-274.PMID: 19681166
- Wang PY, Seabold GK, and Wenthold RJ (2008). Synaptic adhesion-like molecules (SALMs) promote neurite outgrowth. Mol Cell Neurosci. 39:83-94. Epub 2008 Jun 7. PMCID: PMC2602877
- Seabold GK, Wang PY, Chang K, Wang CY, Wang YX, Petralia RS, and Wenthold RJ (2008). The SALM family of adhesion-like molecules forms heteromeric and homomeric complexes. J Biol Chem. 283:8395-8405. Epub 2008 Jan 28. PMCID: PMC2417176
- See PubMed for older publications